HulaMixer™ Sample Mixer from Thermo Fisher Scientific

Product Specs
ItemHulaMixer™ Sample Mixer
CompanyThermo Fisher Scientific
Price Get Pricing Get Quote for this Product
Catalog Number15920D
QuantityEA

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Thermo Fisher Scientific

168 Third Avenue

Waltham, MA 02451

United States

Phone: 800-678-5599

Company Profile

Website: www.thermofisher.com

(1) SAGA Complex Subunit Hfi1 Is Important in the Stress Response and Pathogenesis of Cryptococcus neoformansJ Fungi (Basel)December 15, 2023Chendi K. Yu, Christina J. Stephenson, Tristan C. Villamor, Taylor G. Dyba, Benjamin L. Schulz, James A. Fraserconcentrations of 4%, 1%, 1 mM, and 1%, respectively. Lysates were incubated for 10 min at 4 °C on a HulaMixer Sample Mixer (ThermoFisher Scientific), and then centrifuged at 14,000 rpm at 4 °C for 20 min. The supernatant was

(2) Characterization of markers, functional properties, and microbiome composition in human gut-derived bacterial extracellular vesiclesGut MicrobesDecember 1, 2023Chih-Chi Li, Wei-Fan Hsu, Po-Chieh Chiang, Ming-Che Kuo, Andrew M. Wo, Yufeng Jane Tseng(Abcam) in 200 μL of Ab Binding and Washing Buffer. The mixture was rotated at room temperature using a HulaMixer™ Sample Mixer for 30 minutes. Subsequently, it was washed twice with the same buffer using gentle pipetting. Additionally,

(3) Dynamic interplay between sortilin and syndecan-1 contributes to prostate cancer progressionSci RepAugust 18, 2023Joanna Lazniewska, Ka Lok Li, Ian R. D. Johnson, Alexandra Sorvina, Jessica M. Logan, Carmela Martini, Courtney Moore, Ben S.-Y. Ung, Litsa Karageorgos, Shane M. Hickey, et al.primary or nonspecific IgG isotype control antibodies (Supplementary Table S3) overnight at 4 °C on HulaMixer™ Sample Mixer with rotating angle at 45° and speed at 25 rpm (Thermo Fisher). Lysate-antibody complex was then incubated

(4) New detection method of SARS-CoV-2 antibodies toward a point-of-care biosensorFrontiers in Bioengineering and BiotechnologyJuly 6, 2023Janikua Nelson-Mora, Diana Rubio, Amairani Ventura-Martínez, Luis A. González, Diana Del-Rio, Yuli Aranda-López, Edgar Jiménez-Díaz, Diego Zamarrón-Hernández, Diana G. Ríos-López, Stephanie Aguirre, et al.The mixture was incubated for 24 h at room temperature (RT) under constant agitation in HulaMixer (HulaMixer Sample Mixer, Invitrogen, Carlsbad, United States). Subsequently, two washes were performed with buffer 2 solution

(5) Characteristics of Extracellular Vesicles from a High-Grade Serous Ovarian Cancer Cell Line Derived from a Platinum-Resistant Patient as a Potential Tool for Aiding the Prediction of Responses to ChemotherapyPharmaceuticals (Basel)June 20, 2023Katarina Černe, Nuša Kelhar, Nataša Resnik, Maruša Herzog, Lana Vodnik, Peter Veranič, Borut Kobalsample were added and incubated at 2–8 °C overnight on a HulaMixer (Thermo Fischer Scientific Inc., #15920D). The next day, the assay buffer was added, and the tube was placed in a magnetic separator for 1–2

(6) The deubiquitinase USP11 ameliorates intervertebral disc degeneration by regulating oxidative stress-induced ferroptosis via deubiquitinating and stabilizing Sirt3Redox biologyJune 1, 2023Zhu J, Sun R, Sun K, Yan C, Jiang J, Kong F, Shi J.to the remaining supernatant (total protein content was 1000 μg) and incubated on a turn over-mixer (15920D, Thermo Fisher Scientific, USA) at room temperature for 2 h. The antigen-antibody-magnetic bead complex

(7) Extraction and Oxford Nanopore sequencing of genomic DNA from filamentous ActinobacteriaSTAR ProtocolsMarch 1, 2023Maria Alvarez-Arevalo, Eva Baggesgaard Sterndorff, David Faurdal, Tue Sparholt Jørgensen, Anna-Sophie Mourched, Oliwia Vuksanovic, Subhasish Saha, Tilmann Weber#1851196 Miniplate Spinner Labnet MPS 1000,Sigma-Aldrich,Cat #Z723649 Hula mixer,Invitrogen,Cat #15920D Vortex Mixer – Wise Mix VM 10,WITEG Labortechnik,Cat #WITEG_14002 Labnet 311DS Environmental Shaking Incubator

(8) Critical Role for the Human Cytomegalovirus Major Immediate Early Proteins in Recruitment of RNA Polymerase II and H3K27Ac To an Enhancer-Like Element in Ori LytMicrobiology SpectrumFebruary 14, 2023Eleonora Forte, Ming Li, Fatma Ayaloglu Butun, Qiaolin Hu, Eva Maria Borst, Matthew J. Schipma, Andrea Piunti, Ali Shilatifard, Scott S. Terhune, Michael Abecassis, et al.Biotechnology sc-55492) overnight at 4°C with rotation using a HulaMixer Sample Mixer (Invitrogen, 15920D). Before the other 20 μL of magnetic beads were added to the chromatin-antibody samples, they were first

(9) Whole-genome functional characterization of RE1 silencers using a modified massively parallel reporter assayCell GenomicsJanuary 11, 2023Kousuke Mouri, Hannah B. Dewey, Rodrigo Castro, Daniel Berenzy, Susan Kales, Ryan Tewheyto 500 μL of 20× SSC and added to the reaction followed by agitation on a HulaMixer (Thermo Fisher, 15920D) at room temperature for 15 min. The magnetic beads were then washed once with 1× SSC and twice with

(10) Protocol for large scale whole blood immune monitoring by mass cytometry and Cyto Quality PipelineSTAR protocolsDecember 16, 2022Rybakowska P, Van Gassen S, Martorell Marugán J, Quintelier K, Saeys Y, Alarcón-Riquelme ME, Marañón C.Tube (FACS tube),Fisher Scientific,Cat#352054 HulaMixer Sample Mixer,Thermo Fisher Scientific,Cat#15920D Cytof2 upgraded to HELIOS,Fluidigm,N/A NB injector,Fluidigm,Cat#107018 "HIMAC CT15RE tabletop centrifuges,

(11) Exogenous loading of extracellular vesicles, virus-like particles, and lentiviral vectors with supercharged proteinsCommunications BiologyDecember 1, 2022Koen Breyne, Stefano Ughetto, David Rufino-Ramos, Shadi Mahjoum, Emily A. Grandell, Luís P. de Almeida, Xandra O. Breakefieldprovided by the supplier and the samples were incubated for 24 h at 37 °C or 25 °C, respectively, in a HulaMixer Sample Mixer (Invitrogen). Ni-NTA affinity resin was added and pelleted (16,000 × g for 5 min) to exclude unloaded

(12) Rapid and efficient isolation platform for plasma extracellular vesicles: EV‐FISHERJournal of Extracellular VesiclesNovember 20, 2022Wei‐Lun Pan, Jun‐Jie Feng, Ting‐Ting Luo, Yong Tan, Bo Situ, Rienk Nieuwland, Jing‐Yun Guo, Chun‐Chen Liu, Han Zhang, Jing Chen, et al.EVs The model EVs with 8 × 108/ml were incubated with EV‐FISHER (0.4 mg/ml, 500 μl) for 10 min on a HulaMixer® Sample Mixer at room temperature. Next, the samples were centrifuged (12 800 g, 10 min) and suspended in HEPES buffer

(13) Gallic Acid–Triethylene Glycol Aptadendrimers Synthesis, Biophysical Characterization and Cellular EvaluationPharmaceuticsNovember 14, 2022André Miranda, Roi Lopez-Blanco, Jéssica Lopes-Nunes, Ana M. Melo, Maria Paula Cabral Campello, António Paulo, Maria Cristina Oliveira, Jean-Louis Mergny, Paula A. Oliveira, Eduardo Fernandez-Megia, et al.dialysis was performed in 1 mL of the final volume of buffer solution under constant stirring using an HulaMixer Sample Mixer (Thermo Fisher Scientific, USA). Samples were collected from the bottom tube (100 μL) along the time

(14) Flow cytometric resolution of yeast is affected by enzymatic treatment and culture mediaFEBS Open BioSeptember 1, 2022Mei Wang, Feizhen Wu, Zhongkai Guvortexed. The staining was performed on all the samples on a rotating mixer (HulaMixer; catalog. no. 15920D; Thermo Fisher Scientific) at 4 °C for 24 h. Sonication was not suggested because the refinement of

(15) Comparative efficacy and mechanism of action of cardiac progenitor cells after cardiac injuryiScienceAugust 19, 2022Gunasekaran M, Mishra R, Saha P, Morales D, Cheng WC, Jayaraman AR, Hoffman JR, Davidson L, Chen L, Shah AM, et al.μL) and isotype control antibody for 1 h at 4 °C in HulaMixer Sample Mixer (ThermoFisher Scientific #15920D) Thereafter, cells were centrifuged at 1000 rpm for 5 min and unbound residual CD47 antibody in the

(16) Proteomic and Metabolomic Profiles of T Cell-Derived Exosomes Isolated from Human PlasmaCellsJune 18, 2022Aneta Zebrowska, Karol Jelonek, Sujan Mondal, Marta Gawin, Katarzyna Mrowiec, Piotr Widłak, Theresa Whiteside, Monika Pietrowskathawed samples of CD3(+)exo on the beads, vortexed for 30 sec and then mixed (50 rpm) using a HulaMixer (HulaMixer™ Sample Mixer, Thermo Fisher Scientific, Waltham, MA, USA) for 15 min at 4 °C. The samples of the non-captured CD3(−)exo

(17) Low-Input High-Molecular-Weight DNA Extraction for Long-Read Sequencing From Plants of Diverse FamiliesFrontiers in Plant ScienceMay 19, 2022Alessia Russo et al.samples were then incubated on a HulaMixer™ sample mixer (Thermo Fisher Scientific, MA, United States, 15920D) for 20 min at RT, until the solution was homogenised. Bead clean-up was performed with two washing

(18) Light triggered nanoscale biolistics for efficient intracellular delivery of functional macromolecules in mammalian cellsNature CommunicationsApril 14, 2022Juan C. Fraire, Elnaz Shaabani, Maryam Sharifiaghdam, Matthias Rombaut, Charlotte Hinnekens, Dawei Hua, Jana Ramon, Laurens Raes, Eduardo Bolea-Fernandez, Toon Brans, et al.solution was mixed with 1 ml of carboxylated-NPs and the product was shaken for 24 h at 200 rpm in a HulaMixer® Sample Mixer (Life Technologies, Waltham, USA), protected from light at room temperature. The functionalized NPs

(19) Uptake, functionality, and re-release of extracellular vesicle-encapsulated cargoCell ReportsApril 12, 2022Killian O’Brien, Stefano Ughetto, Shadi Mahjoum, Anil V. Nair, Xandra O. Breakefieldworking concentration of 1% in PBS and incubated with samples for 30 min with gentle agitation on a HulaMixer™ Sample Mixer (Thermofisher) at RT. Proteinase K (Qiagen) treatment was performed at a final concentration of 100

(20) Using genetically modified extracellular vesicles as a non-invasive strategy to evaluate brain-specific cargoBiomaterialsFebruary 1, 2022David Rufino-Ramos, Sevda Lule, Shadi Mahjoum, Stefano Ughetto, D. Cristopher Cristopher Bragg, Luís Pereira Pereira de Almeida, Xandra O. Breakefield, Koen BreyneHEK293T cells were incubated with magnetic beads at room temperature over 1 h on a HulaMixer (Invitrogen, 15920D) and then the cell-bound magnetic beads were isolated by using a magnetic rack, DynaMag-2 Magnet (Invitrogen). 4.5.

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